Cultural, morphological and physiological characterization of Fusarium spp. in pitahaya, Palora, Ecuador
DOI:
https://doi.org/10.35381/a.g.v7i12.4442Keywords:
Fusarium, characterization, yellow dragon fruit, Ecuador, (AGROVOC Thesaurus).Abstract
The objective of this work was to determine the cultural, morphological and physiological in vitro characterization of Fusarium spp. the causal agent of vascular wilt of yellow pitahaya (Hylocereus spp) in the Amazon region of Ecuador. Roots were collected from 8 farms in the Palora canton. Isolations were evaluated and identified by cultural, morphological and physiological characterization. Semi-solid culture media (PDA and CLA) and PDB liquid culture media were used for the tests. The radial growth rate was 2.8 mmxday-1 of the isolates, regardless of their origin. No significant differences were found in the cultural characterization in terms of colony border and texture among the isolates. Variations were detected in their coloration, with red, pale red, yellowish red to light pink colors. No significant differences were observed in the size of microconidia, macroconidia and conidiophores, determining that pH 7 is the optimum for Fusarium spp. Development.
Downloads
References
Baldrian, P., y Gabriel, J. (2002). Intraspecific variability in growth response to cadmium of the wood-rotting fungus Piptoporus betulinus. Mycologia, 94(3), 428-300. https://pubmed.ncbi.nlm.nih.gov/21156514/
Bastidas, O. (2009). Technical Note-Neubaver Chamber Cell Counting. https://n9.cl/wfxp8
Chandran, R., y Kumar, R. (2012). Studies on cultural, morphological variability in isolates of Fusarium solani (Mart.) Sacc., incitant of dry root-rot of Citrus. Current Biotica, 6(2), 152-162. https://n9.cl/vppv5l
Chehri, K., Salleh, B., y Zakaria, L. (2014). Morphological and Phylogenetic Analysis of Fusarium solani Specie Complex in Malaysia. Microbial Ecology, 69(3), 457-471. https://doi.org/10.1007/s00248-014-0494-2
Dubey, S. C., Shingh, S. R., y Shingh, B. (2010). Morphological and pathogenic variability of India isolates of Fusarium oxysporum f.sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection, 43(2), 174-190. https://doi.org/10.1080/03235400802021108
Duarte Leal, Y., Echevarría Hernández, A., y Martínez Coca, B. (2016). Identificación y caracterización de aislamiento de Fusarium spp. presentes en garbanzo (Cicer arietinum L.) en Cuba. Revista Protección Vegetal, 31(3), 173-183. https://n9.cl/9k0gg
González, I., Arias, Y., y Peteira, B. (2012). Aspectos generales de la interacción Fusarium oxysporum f. sp. Lycopersici–tomate. Revista de Protección Vegetal, 27(1), 1-7. https://n9.cl/eae6n
He, P., Ho, H., Wu, H. M., y He, Y. (2012). Bipolaris cactivora causing fruit rot of dragon fruit imported from Vietnam. Plant pathology y Quarantine, 2(1), 31-35. https://doi.org/10.5943/ppq/2/1/5
Hunt, D., Taylor, N., y Graham Ch. (2006). The New Cactus Lexicon. DH Books. https://n9.cl/nub3s
Kawuri, R., Suprapta, D. N., Nitta, Y., y Homma, T. (2012). Destructive Leaf Rot Disease Caused by Fusarium oxysporum on Aloe barbadensis Miller in Bali. Agricultural Science Research Journal, 2(6), 295-301. https://n9.cl/jlzifi
Leslie, J. F., y Summerell, B. A. (2006). The Fusarium Laboratory Manual. Blackwell Editorial. https://doi.org/10.1002/9780470278376
Lezcano, J., Martínez, B., y Alonso, O. (2012). Caracterización cultural y morfológica e identificación de diez aislamientos de Fusarium procedentes de semillas de Leucaena leucocephala cv. Perú. Pastos y Forrajes, 35(2), 187-196. https://n9.cl/zig9z
Madhukeshwara, S., y Seshadri, V. S. (2001). Varation and management of Fusarium Wilt of Pigeon Pea (Cajanus cajan (L.). Tropical Agricultural Research, 13, 380-394. https://n9.cl/ha9c4
Nelson, P. E., Tousson, T. A., y Marasas, W. F. O. (1983). Fusarium species: An Illustrated Manual for Identification. Pennsylvania State University Press. https://n9.cl/opazx
Ochoa J., Fonseca G., y Ellis, M. (2000). First report of Fusarium wilts of babaco of Babaco (Carica x heilbornii var. pentagona) in Ecuador. Plant Disease, 84(2), 199. https://doi.org/10.1094/PDIS.2000.84.2.199A
Orozco de Amezquita, M., Garcés de Granada, E., y Arbelaéz, G. (1993). Efecto de diferentes niveles de nitrógeno, potasio y pH en el desarrollo de Fusarium oxysporium f.sp. dianthi, agente causal del marchitamiento vascular del clave. Agronomía colombiana, 10(1), 90-102. https://n9.cl/xd1ge6
Ratankumar, A., Susheel, K., Vishambhar, D., Sudip, K., Singh, S., Sumitra, T., Boopathi, L., Saurav, S., Priyanka, I., Sushanti, T., Emekamwamut, N. (2023). Fusarium equiseti as the pathogen causing stem rot disease of red-fleshed dragon fruit (Hylocereus polyrhizus) in India. Crop Protection, 173, 106380. https://doi.org/10.1016/j.cropro.2023.106380
Rodríguez Velásquez, E. (2013). Caracterización de aislamientos de Fusarium spp. obtenidos de zonas productoras de uchuva (Physalis peruviana) en Cundinamarca y Boyacá. [Tesis de Maestría; Universidad Nacional de Agronomía], Repositorio Institucional UNAL. https://n9.cl/wjqvi
Ríos, G., Villavicencio, A., Guevara, V., Pichardo, S. (1997). Estudio epidemiológico y etiológico de la bacteriosis de la pitahaya Hylocereus undatus Britton and Rose, Smith y su efecto sobre variedades del cultivo. [Trabajo de grado, Universidad Nacional Agraria] Repositorio Institucional RiUNA. https://n9.cl/gdik5
Salazar González, C., Serna Cock, L., & Gómez López, E. (2016). Molecular characterization of Fusarium associated with basal rot of thefruit of pitahaya (Selenicereus megalanthus). Agronomía Mesoamericana, 27(2), 277-285. https://doi.org/10.15517/am.v27i2.21269
Schroers, H. J., O’Donnell, K., Lamprecht, S. C., Kammeyer, P. L., Johnson, S., Sutton, D. A., Rinaldi, M. G., Geiser, D. M., & Summerbell, R. C. (2009) Taxonomy and phylogeny of the Fusarium dimerum species group. Mycologia, 101(1), 44-70. https://doi.org/10.3852/08-002
Sosa Sánchez, O., Romero Bastidas, R., Silva Pupo, J., Gaibor Fernández, R., Rodríguez Rodríguez, R, & Ceiro Catasú, W. (2022). Caracterización cultural-morfométrica como herramienta para la identificación de Fusarium spp., asociado a Nicotiana tabacum L. Agronomía Costarricense, 46(1),111-128, 2022. https://doi.org/10.15517/rac.v46i1.49872
Suárez Román, R. S. (2012). Caracterización morfoanatómica y fisiológica de semilla sexual de pitahaya amarilla Selenicereus megalanthus (Haw.) Britt & Rose. Revista de la Asociación Colombiana de Ciencias Biológicas, 1(24), 97-111. https://n9.cl/vlu2w
Vargas, Y., Pico, J., Díaz, A., Sotomayor, D., Burbano, A., Caicedo, C., Paredes, N., Congo, C., Tinoco, L., Bastidas, S., Chuquimarca, J., Macas, J., & Viera, W. (2020). Manual Técnico del cultivo de pitahaya. INIAP. https://n9.cl/3rx4p
Wiwik, S. R., Suprapta, D. N., Sudana, M., & Swantara, M. D. (2013). First report on Fusarium solani, a pathogenic fungus causing stem rot disease on dragon fruits (Hylocereus sp.) in Bali. J. Biol. Agric. Healthcare 3, 93-99. https://n9.cl/sbvdt
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Karina María Elena Carrera-Sánchez , Edison Roberto Suntasig-Negrete , Paul Marcelo Manobanda-Pinto , Javier Dominguez-Brito
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
CC BY-NC-SA : Esta licencia permite a los reutilizadores distribuir, remezclar, adaptar y construir sobre el material en cualquier medio o formato solo con fines no comerciales, y solo siempre y cuando se dé la atribución al creador. Si remezcla, adapta o construye sobre el material, debe licenciar el material modificado bajo términos idénticos.
OAI-PMH URL: https://fundacionkoinonia.com.ve/ojs/index.php/agroecologiaglobal/oai
.png)
